Data items in the EM_CLASSES category record details about the particle classification. Particle classification involves grouping images that are similar, and separating images that are distinct. In practical use, this means that experimental projections that have the same orientation (shape) are placed within the same category for later averaging. In this case, orientation means that the particles are showing the same face to the viewer and the only difference between them is that they can be rotated by some angle in the plane of the image. The experimental projections might also be shifted relative to each other, but the centering of the experimental projections is often done before classification. is this required? E(e1,e2,e3) = E(w,h,i) cos(i)cos(h)cos(w)-sin(i)sin(h) cos(i)cos(h)sin(w)+sin(i)sin(h) -cos(i)sin(h) -sin(i)cos(h)cos(w)-cos(i)sin(h) sin(i)cos(h)sin(w)+cos(i)sin(h) sin(i)sin(h) sin(h)cos(w) sin(h)sin(w) cos(h)