Data Item _em_sample_preparation.details
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General 
- Item name
- _em_sample_preparation.details
- Category name
- em_sample_preparation
- Attribute name
- details
- Required in PDB entries
- no
- Used in current PDB entries
- No
Item Description
Details of the specimen preparation
Item Example1
Detergent-solubilized particles eluted from the cation-exchange
column were directly adsorbed for 1 min to parlodion carbon-coated
copper grids rendered hydrophilic by glow discharge at low pressure
in air. Grids were washed with 4 drops of double-distilled water
and stained with 2 drops of 0.75% uranyl formate.
Item Example2
Selectively stained by injection of horseradish peroxidase, embedded in Spurr's resin and cut into 2-3 um thick sections.
Item Example3
S. cerevisiae PDC was purified to near homogeneity from baker's yeast by modification of a published procedure. Highly purified E1 was obtained by resolution of PDC with 2 M NaCl at pH 7.3 followed by FPLC on a Superdex 200 column. The weight-average molecular weight of the PDC was determined by light scattering measurement to be ~8 x 106. On the basis of the known molecular weight of the complex and its component enzymes and the experimentally determined polypeptide chain ratios of E2/BP/E3, we estimated that the subunit composition of the S. cerevisiae PDC is ~24 E1 tetramers, 60 E2 monomers, 12 BP monomers, and 8 E3 dimers. Sufficient E1 was added to a sample of the PDC preparation to increase the molar ratio of E1/E2 core to 60:1.
Item Example4
embedded in vitreous ice.
Data Type 
- Data type code
- text
- Data type detail
- text item types / multi-line text ...
- Primitive data type code
- char
- Regular expression
- [][ \n\t()_,.;:"&<>/\{}'`~!@#$%?+=*A-Za-z0-9|^-]*
Related Items 
| Related Item Name | Relation Type |
|---|---|
| _em_specimen.details | replacedby |